Our work adds 46 species to the 43 previously known from Borneo, bringing the total for the island to 89. Three new species are described: Bactrocera (Bactrocera) melanobivittata Doorenweerd, sp. nov., Dacus (Mellesis) danumensis Doorenweerd, sp. nov., and Zeugodacus (Zeugodacus) cataracta Doorenweerd, sp. nov.
Adult Bactrocera fruit fly ID provides identification support for 800 species of fruit flies in the economically important genus Bactrocera and its closely allied genera in the tribe Dacini. This website includes fact sheets and a Lucid multi-entry key with color illustrations and photos.
We present CCSconsensuser, an end-to-end pipeline that generates consensus sequences from amplicon sequencing using high-fidelity reads produced by PacBio circular consensus sequencing (CCS).
We generated a COI reference library for 265 species of Dacini containing 5601 sequences that span most of the COI gene using circular consensus sequencing. We compared distance metrics versus monophyly assessments for species identification and although we found a ‘soft’ barcode gap around 2% pairwise distance, the exceptions to this rule dictate that a monophyly assessment is the only reliable method for species identification.
We present restriction site-associated DNA sequencing (RAD-seq) genomic data for 2,292 specimens, which unequivocally supports the delimitation of two new species, here described as Bactrocera borneoensis sp. n. Doorenweerd & San Jose and B. incognita sp. n. Doorenweerd & San Jose.
Under concatenation, both datasets suggest identical species relationships with mostly high statistical support. However, multispecies coalescent and multispecies network approaches suggest markedly different hypotheses and detected significant gene flow.
As the amount of genomic data for nonmodel taxa grows, it is increasingly clear that gene flow across species barriers in insects is much more common than previously thought.